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KMID : 0903619980390050641
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Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 5 p.641 ~ p.646
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Effects of Culture Conditions on Dormant Status of In Vitro Regenerated Lilium Oriental Hybrid Bulblets
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Abstract
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This study was performed to investigate the effect of culture conditions on dormancy status of in vitro bulblets of Lilium Oriental hybrid ´Casablanca´ and ´Marcopolo´ . The regenerated bulblets were dormant regardless of culture period and sucrose concentration. Non-dormant bulblets were produced much larger at lower temperatures than 15℃ : 100℃ in ´Casablanca´, but were 50℃ and 70℃ at 10℃ and 15℃, respectively in ´Marcopolo´. GA₃ was effective for production of non-dormant bulblet in vitro. This effect was greater in ´Marcopolo´ than ´Casablanca´. High concentration (9-12%) of sucrose in culture medium was effective for production of leafless bulblets and for bulblet growth. Dormancy of bulblets obtained in vitro was breaked completely by chilling at 4℃ for 6 weeks. But the chilling-treated bulblets were not bolted regardless of light and planting depth.
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